PDF Publication Title:
Text from PDF Page: 008
International Journal of Photoenergy 7 5β 4 3 2 1 0 Hold only Figure 4: TUNEL+ cell count in dim-reared animals. Animals raised in dim light (no light damage) treated with 90 J/cm2 670 nm light had three times more TUNEL+ cells compared to control animals that were hand-held only for 30 minutes to mimic treatment conditions (π = 3 animals for each group, βββdenotes significance π < 0.05; error bars represent SEM). 150 100 50 β 80 60 40 20 β β β β LD only 90J/cm2 only 00 ATP SRC PL BR NMR LD only 670 nm light + LD (a) Mitochondrial respiration Non-LD 670 nm light only 670 nm light + LD in a thicker ONL, compared to all other treatment groups and controls exposed to high intensity damage. Figures 3(b)β3(e) show the effects of four different 670 nm light doses in animals exposed to βhighβ intensity light damage. The data show largely comparable ONL thickness in the light damage controls and animals treated with 9, 18, or 36 J/cm2 670 nm light (Figures 3(b)β3(d)) but a significantly thicker ONL adjacent to the optic nerve head in animals treated with 90 J/cm2 red light (asterisks Figure 3(e)). 3.1.4. 90 J/cm2 670 nm Effect on Normal Retinas. Animals reared in dim light conditions, not subjected to light damage or 670 nm light treatment, had virtually no TUNEL+ cells in the retina (control, Figure 4). Dim-reared animals treated with 90 J/cm2 670 nm light had low numbers of TUNEL+ cells in the ONL, although these numbers were significantly higher than in the control dim-reared animals (90 J/cm2 only, Figure 4). 3.2. Effect of 670nm Light on Mitochondrial Metabolism. Light damaged 661W photoreceptor cells treated with 670 nm light exhibited a significant increase in spare respira- tory capacity, compared to control cells (light damage only) (Figure 5(a)). In addition to a change in spare respiratory capacity, there was also an increase in nonmitochondrial res- piration in treated cells compared to control cells. However, there was no change in ATP production, proton leak, or basal respiration. No changes were seen in the measurements of basal extracellular acidification rate (ECAR) between 670 nm light treated cells and nonlight damaged cells (Figure 5(b)). Light damaged cells that had been treated with 670nm light β Figure 5: Effect of 9 J/cm2 of 670 nm light on the mitochondrial respiration of 661 W photoreceptor cells. (a) Under light damage conditions, the cells exhibited an increase in both spare respiratory capacity (SRC) and nonmitochondrial respiration (NMR) when treated with 670 nm light. ATP production (ATP), proton leak (PL), and basal respiration (BR) did not change between groups (π = 4 for both groups; βββ denotes significance, π < 0.05; error bars represent SEM). (b) 670 nm light did not produce a difference in relative basal ECAR measurements in both normal and light damaged conditions. Light damage (LD) significantly decreased the ECAR measurements in cells, both treated and nontreated, when compared to nonlight damaged cells (π = 3 for nonlight damaged cells; π = 4 for light damaged cells; βββ denotes significance using one-way ANOVA with Tukeyβs post hoc test, π < 0.05; error bars represent SEM). (b) Relative glycolytic rate Oxygen consumption rate (pmol/min) Extracellular acidification rate (mpH/min) TUNEL+ cell countPDF Image | 670 nm Light Therapy to Protect vs Photoreceptor Cell Death
PDF Search Title:
670 nm Light Therapy to Protect vs Photoreceptor Cell DeathOriginal File Name Searched:
Efficacy_of_670_nm_Light_Therapy_to_Protect_agains.pdfDIY PDF Search: Google It | Yahoo | Bing
Cruise Ship Reviews | Luxury Resort | Jet | Yacht | and Travel Tech More Info
Cruising Review Topics and Articles More Info
Software based on Filemaker for the travel industry More Info
The Burgenstock Resort: Reviews on CruisingReview website... More Info
Resort Reviews: World Class resorts... More Info
The Riffelalp Resort: Reviews on CruisingReview website... More Info
| CONTACT TEL: 608-238-6001 Email: greg@cruisingreview.com | RSS | AMP |