PDF Publication Title:
Text from PDF Page: 003
Appl. Sci. 2020, 10, 4290 3 of 9 Figure 1. Effect of light sources on photoactive+-mediated aPDT on E. faecalis (CFU/mL). R1: λ = 635 nm, R2: λ = 660 nm, blue: λ = 420–480 nm, * p < 0.05; significantly different from the control. 2.3. Antimicrobial Photodynamic Therapy of Bacterial Suspensions The antimicrobial effect of Photoactive+ against E. faecalis was determined by the broth microdilution method as recommended by the clinical and laboratory standards institute (CLSI) [15]. 150 μL of bacterial suspension at a final concentration of 1.5 × 108 CFU/mL was transferred to each well of a 96-well round-bottomed sterile polystyrene microplate (TPP; Trasadingen, Switzerland). The Photoactive+ solution (150 μL) was added to each well. The final concentration of Photoactive+ was 0.01 g/mL. The microplates were incubated for 5 min in the dark at room temperature at 25 ± 2 ◦C. The wells were then exposed to light sources with different wavelengths and exposure times as listed in Table 1. 2.4. Colony Count Assessment Table 1. Experimental groups. Groups R1 R2 Blue R1 + Photoactive+ R2 + Photoactive+ Blue + Photoactive+ Blue–R1 Blue-R2 R1–Blue Re2–Blue Photoactive+ Control Wavelength (λ nm) 635 660 420–480 635 660 420–480 First 420–480, then 635 First 420–480, then 660 First 635, then 420–480 First 660, then 420–480 Power/Power Density mW/(mW/cm2 ) 220 150 1000–1400 220 150 1000–1400 1000–1400 + 220 1000–1400 + 150 220 + 1000–1400 150 + 1000–1400 Irradiation Time (s) Only photosensitizer without No treatment After treatment 10 μL of each well was transferred to the wells that contained 100 μL BHI broth and serial diluted 10-fold stepwise from column one to column five. 10 μL of each dilution was cultured on BHI agar plates (Merck, Darmstadt, Germany) and incubated for 24 h at 37 ◦C. Microbial viability assay was performed, according to a previously reported method [16]. The number of colony forming units (CFU)/mL was calculated using Miles and Misra method [17]. 60 88 22 60 88 22 11 + 30 11 + 44 30 + 11 44 + 11 irradiation 180 264 66 180 264 66 33 + 90 33 + 132 90 + 33 132 + 33PDF Image | Antimicrobial Photodynamic Therapy Chlorophyllin–Phycocyanin
PDF Search Title:
Antimicrobial Photodynamic Therapy Chlorophyllin–PhycocyaninOriginal File Name Searched:
applsci-10-04290-v2.pdfDIY PDF Search: Google It | Yahoo | Bing
Cruise Ship Reviews | Luxury Resort | Jet | Yacht | and Travel Tech More Info
Cruising Review Topics and Articles More Info
Software based on Filemaker for the travel industry More Info
The Burgenstock Resort: Reviews on CruisingReview website... More Info
Resort Reviews: World Class resorts... More Info
The Riffelalp Resort: Reviews on CruisingReview website... More Info
| CONTACT TEL: 608-238-6001 Email: greg@cruisingreview.com | RSS | AMP |