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Appl. Sci. 2020, 10, x 6 of 10 Appl. Sci. 2020, 10, 4290 5 of 9 The peak absorption of Photoactive+ which assessed by company was shown in Figure 2a (Source: https://www.wmedicalsystems.com/) and our assessment confirmed its absorption in red (620–660 nm) and blue (400–450 nm) region of electromagnetic spectrum (Figure 2b). Figure 2. (a) Absorption curve of chlorophyllin and phycocyanin. (b) Absorption curve of Photoactive+. The results of MTT assay for Photoactive+ at concentration of 0.01-g/mL showed that after the Figure 2. (a) Absorption curve of chlorophyllin and phycocyanin. (b) Absorption curve of incubation period, there was no significant reduction in the number of viable cells compared to the Photoactive+. control group (p > 0.05; Figure 3a). Additionally, morphologically, HuGu of the control group showed a spindle-shaped cell and those in contact with the Photoactive+ demonstrated the typical stellate The results of MTT assay for Photoactive+ at concentration of 0.01-g/mL showed that after the appearance of this type of cell (Figure 3b,c). incubation period, there was no significant reduction in the number of viable cells compared to the control group (p > 0.05; Figure 3a). Additionally, morphologically, HuGu of the control group showed a spindle-shaped cell and those in contact with the Photoactive+ demonstrated the typical stellate appearance of this type of cell (Figure 3b,c).PDF Image | Antimicrobial Photodynamic Therapy Chlorophyllin–Phycocyanin
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