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Biphasic dose response in low level light therapy FIGURE 10. Biphasic dose response in generation of ROS as detected by fluorescence probe under same conditions as Fig 9 but measured at 5 minutes post-irradiation. et al. 2002; Lubart et al. 2005; Pal et al. 2007). We used dichlorodihydro- fluorescein diacetate (DCHF-DA) which is taken up into cells, hydrolyzed and oxidized to a fluorescent form by most species of ROS probably via lipid peroxides (Diaz et al. 2003). As can be seen in Figure 10 even the low fluence of 0.003 J/cm2 produced detectable levels of ROS, greater at 0.03 J/cm2 and maximum at 0.3 J/cm2 with a slight decrease observed at 3 J/cm2. The maximum level observed at 0.3 J/cm2 was only slightly less than that observed inside the cells after addition of hydrogen peroxide to the extracellular medium. 4.2. Mouse wound healing In an in vivo study (Demidova-Rice et al. 2007) we used a set of flu- ences of 635-nm (+/–15-nm) light delivered from a filtered lamp. The model was a full thickness dorsal excisional wound in BALB/c mice treat- ed with a single exposure to light 30 minutes after wounding. These flu- ences were 1, 2, 10 and 50 J/cm2 delivered at constant fluence rate of 100 mW/cm2 and taking 10, 20, 100 and 500 seconds respectively. In this model the untreated wound tends to expand for 2-3 days after it was made, but even a brief exposure to light soon after wounding, reduces or stops the expansion of the wound and the integrated time course of the wound size can therefore be significantly reduced. Our hypothesis is that fibroblasts in the edge of the wounded dermis can be transformed into myofibroblasts, and the contractile nature of these cells with their smooth muscle actin fibers prevents the wound expanding. It should be noted that the fibroblast-myofibroblast transition can be mediated by NF-κB activation (Watson et al. 2008). As shown in Figure 11 there was a bipha-PDF Image | BIPHASIC DOSE RESPONSE IN LOW LEVEL LIGHT THERAPY
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