PDF Publication Title:
Text from PDF Page: 003
oped LEDs to offer an effective alternative to lasers. These diodes can be configured to produce multiple wavelengths, can be arranged in large, flat arrays (allowing treatment of large wounds), and produce no heat. It is also of importance to note that LED light therapy has been deemed a nonsignificant risk by the FDA; thus, FDA approval for the use of LEDs in humans for light therapy has been obtained. NASA LEDs stimulate the basic energy processes in the mi- tochondria (energy compartments) of each cell, particularly when near-infrared light is used to activate the wavelength sen- sitive constituents inside (chromophores, cytochrome systems). Optimal light wavelengths (proven in prior studies of laser and LED light)2,3,8,11–14,17,18 to speed wound healing include 680, 730, and 880 nm. These wavelengths can be produced accu- rately by NASA LEDs, which have a bandwidth of 25 nm. The depth of near-infrared light penetration into human tissue has been measured spectroscopically.2,3,19 Spectra taken from the wrist flexor muscles in the forearm and muscles in the calf of the leg demonstrate that most of the photons at wavelengths of 630–800 nm travel approximately 23 cm through the skin sur- face (light input) and muscle, exiting at the photon detector. Data collection and cataloging to elucidate the absorption coef- ficients of the various human tissues are currently underway by the principle investigator. LED IN VITRO STUDIES In order to understand the effects of LEDs on cell growth and proliferation, we have measured radiolabeled thymidine incor- poration in vitro in several cell lines treated with LED light at various wavelengths and energy levels. As previously reported, 3T3 fibroblasts (mouse-derived skin cells) responded extremely well to laser and LED light exposure.8,10–13,20,21,22 Cell growth increased 150–200% over untreated controls. Additionally, os- teoblasts (rat derived) have been reported to have increased DNA synthesis and increased cellular growth rate when ex- posed to laser light.23 With LED treatment, we have found that these cells demonstrated a growth-phase specificity, responding only when cells are in the growth phase. In these experiments, fibroblasts seeded at a concentration of 500 cells/well and os- teoblasts, seeded at a concentration of 1 3 103 cells/well, were 307 NASA LED Irradiation and Wound Healing FIG. 3. Growth phase specificity of L-6 skeletal muscle cells treated with individual wavelengths at 8 J/cm2; 50 mW/cm2. FIG. 4. LED response at 4 J/cm2, 50 mW/cm2 using individual wavelengths of 670, 728, and 880 nm (percentage change from control versus number of hours after LED treatment).PDF Image | Effect of NASA Light-Emitting Diode Irradiation on Wound Healing
PDF Search Title:
Effect of NASA Light-Emitting Diode Irradiation on Wound HealingOriginal File Name Searched:
Effect-of-NASA-light-emitting-diode-irradiation-on-wound-healing.pdfDIY PDF Search: Google It | Yahoo | Bing
Cruise Ship Reviews | Luxury Resort | Jet | Yacht | and Travel Tech More Info
Cruising Review Topics and Articles More Info
Software based on Filemaker for the travel industry More Info
The Burgenstock Resort: Reviews on CruisingReview website... More Info
Resort Reviews: World Class resorts... More Info
The Riffelalp Resort: Reviews on CruisingReview website... More Info
CONTACT TEL: 608-238-6001 Email: greg@cruisingreview.com (Standard Web Page)