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MATERIALS & METHODS Growth Conditions for Tropism Studies This study used wild-type (WT) Arabidopsis thaliana seeds of ecotypes Landsberg (Ler) and Columbia (Col). Before surface sterilizing the seeds, 100 x 100 x 15-mm gridded square petri dishes was prepared. Sterilized agar consisting of 1.2% (w/v) Arabidopsis growth medium bacto-agar, as described by Kiss et al. (1997), half-strength Murashige and Skoog salts medium, and 1% (w/v) sucrose at pH 5.5 was poured into the plates. Once the agar solidified, a sterilized nitrocellulose film (Promega, V7131) was laid on top of the agar as a membrane. Prepared plates were double-wrapped in Parafilm and chilled for 24 hours at 4oC. The seeds were surface sterilized under a laminar flow hood via rinsing with multiple ethanol and Triton X-100 concentrations. The first rinse was 70% (v/v) ethanol and 0.002% (v/v) Triton X-100 solution for 5 minutes. Next, seeds were given 2 rounds of rinsing by 95% (v/v) ethanol, each rinse lasting for 1 minute. Seeds were then rinsed given a 1 minute rinse with 0.01% (v/v) Triton X-100 solution before undergoing 4 consecutive sterilized water rinses. Seeds were sown onto the plate membranes with 2 rows of 6 seeds per plate. Plates were once again wrapped in two layers of Parafilm and subjected to a 24-hour cold treatment at 4oC. Eight plates were made for each ecotype. !xiPDF Image | EFFECTS OF RED LIGHT ON BLUE LIGHT-BASED PHOTOTROPISM
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