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2.3.3 Statistics ............................................................................................................. 40 2.4 2.4.3 2.5 Discussion ............................................................................................. 49 2.6 Conclusion ............................................................................................. 51 2.7 References ............................................................................................. 52 Chapter 3 Effects of 670 nm irradiation on sensorimotor function following mild-moderate hemicontusion spinal cord injury ....................................................................................55 3.1 Abstract ................................................................................................. 56 3.2 Introduction .......................................................................................... 56 3.3 Methods ................................................................................................. 58 3.3.1 Hemicontusion spinal cord injury .......................................................................... 58 3.3.2 Treatment and experimental groups..................................................................... 58 3.3.3 Light penetration ................................................................................................. 58 3.3.4 Temperature measurement .................................................................................. 59 3.3.5 Sensitivity assessment ......................................................................................... 59 3.3.6 Somatosensory assessment ................................................................................. 60 3.3.7 Locomotor assessment......................................................................................... 61 3.3.8 Immunohistochemistry and TUNEL ...................................................................... 61 3.3.9 Statistics ............................................................................................................. 62 3.4 Results................................................................................................... 63 3.4.1 Red light penetrates the spinal cord...................................................................... 63 3.4.2 Surface temperature changes following light treatment........................................ 64 3.4.3 Red light reduces allodynia following spinal cord injury ......................................... 64 3.4.4 Red light improves sensory conduction through dorsal column pathways .............. 66 3.4.5 Red light improves locomotor recovery ................................................................. 67 3.4.6 Red light reduces cell death at the injury zone....................................................... 68 3.4.7 Red light reduces total activated microglia/macrophages but promotes the expression of the anti-inflammatory/wound healing (M2) subtype......................................................... 69 Results................................................................................................... 40 670 nm LED at 100 mW/cm2 can penetrate tissues up to 50 mm thick .................... 40 670 nm LED penetrates better in tissues with bone component.............................. 47 2.4.1 2.4.2 670 nm LED penetration is not affected by skin tone ............................................. 42 viPDF Image | Effects of Red Light Treatment on Spinal Cord Injury
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