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3.5 Discussion.............................................................................................. 72 3.6 Conclusion ............................................................................................. 75 3.7 References ............................................................................................. 76 Chapter 4 670 nm LED reduces mechanical sensitivity associated with reduced astrocytic reactivity and iNOS expression in microglia/macrophages following spinal cord injury in rats ................................................................................ 79 4.1 Abstract ................................................................................................. 80 4.2 Introduction........................................................................................... 80 4.3 Methods ................................................................................................. 81 4.3.1 Spinal cord injury.................................................................................................. 81 4.3.2 Treatment............................................................................................................ 82 4.3.3 Sensitivity testing................................................................................................. 82 4.3.4 Immunohistochemistry......................................................................................... 83 4.3.5 Image acquisition and quantification .................................................................... 83 4.3.6 Statistical analysis ............................................................................................... 83 4.4 Results ................................................................................................... 84 4.4.1 4.4.2 Red light reduces the incidence of developing hypersensitivity up to 5 days but without reducing mechanical sensitivity of hypersensitive animals ....................................................88 4.4.3 Red light reduces mechanical sensitivity in animals that do not develop hypersensitivity ...................................................................................................................92 4.4.4 Red light reduces astrocyte reactivity after 1-dpi in the ipsilateral spinal cord.........94 4.4.5 Red light does not affect IL1β expression in astrocytes........................................... 97 4.4.6 Red light does not affect IL1β expression in microglia/macrophages..................... 100 4.4.7 Red light reduces iNOS expressing microglia/macrophages at the injury zone ...... 102 Red light reduces mechanical hypersensitivity.......................................................84 4.5 Discussion............................................................................................ 104 4.6 Conclusion ........................................................................................... 108 4.7 References ........................................................................................... 108 Chapter 5 General discussion ............................................... 112 5.1 Penetration of 670 nm LED................................................................ 113 viiPDF Image | Effects of Red Light Treatment on Spinal Cord Injury
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