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CHAPTER 4 all levels at individual time point (black lines, CLMM) and between 2 groups at different levels (grey lines, CLMM) are indicated. Data is expressed as mean ± SEM as per Figure 4.1a inset; n values indicated for each group; * p < 0.05, ** p < 0.01, p value indicated for 0.1 < p < 0.05. See Figure 4.1 for abbreviations. 4.4.2 Red light reduces the incidence of developing hypersensitivity up to 5 days but without reducing mechanical sensitivity of hypersensitive animals Not all animals develop hypersensitivity following mild spinal cord injury. Animals were therefore separated into hypersensitive (CSS > hypersensitivity threshold) and normosensitive populations (CSS ≤ hypersensitivity threshold). Figure 4.3 shows the percentage of hypersensitive animals in both spinal cord injured animals (Figure 4.3a) and sham-injured animals (Figure 4.3b). Following injury, over half of the SCI group developed hypersensitivity up to 5-dpi, and only approximately 1/3 in the SCI+670 group. While the effect of red light significantly reduced the incidence of developing hypersensitivity over the 1 to 5-dpi period (p = 0.030), the effect just failed to reach significance across the entire 7-day period (p = 0.058). Red light also failed to demonstrate a significant effect on sham-injured animals developing hypersensitivity (Figure 4.3b; p = 0.061), note however, that only a small number in this group became hypersensitive (see Figure 4.4 for n values). In spinal cord injured animals that developed hypersensitivity (Figure 4.3c, see Figure 4.4 for n values), no significant overall effects on CSSs of time (p = 0.3420, LMER) or 670 nm light treatment (p = 0.23, LMER) were detected. Only 1 or 2 sham-injured animals developed hypersensitivity at each time point (Figure 4.3d, see Figure 4.4 for n values). 88PDF Image | Effects of Red Light Treatment on Spinal Cord Injury
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