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indexes compared to FL were theoretically transformed from the average value when the value of each index of FL was assumed to be 100% (Supplement Data Table S2). Then, the total average of influence of each LED light on A. roxburghii plants was calculated from nine transformed data. Interestingly, we have found that the highest total average influence was in the BR treatment, while a slight inhibition was in the red treatment. All other light treatments showed negligible effects Plants 2020, 9, 1344 5 of 15 compared with the FL (Table 1; Figure 4). Figure 3. Effects of light condition on A. roxburghii leaves area. (a): fluorescent light; (b): red; (c): blue; Figure 3. Effects of light condition on A. roxburghii leaves area. (a): fluorescent light; (b): red; (c): blue; (d): BR; (e): BRW151; (f): BRW142. Scale bars = 1 cm. (d): BR; (e): BRW151; (f): BRW142. Scale bars = 1 cm. The stem cell anatomy of this plant was significantly affected by the LEDs. Specifically, under the blue light, the highest cell width value was detected (36.87 μm), followed by BRW151 (33.11 μm) and BR (30.31 μm), while the lowest value of cell width was found under the red light (25.37 μm) and BRW142 (27.64 μm), which was lower than that of FL (28.58 μm). Interestingly, remarkable differences in cell length were found under the red and BR lights of which red light immensely promoted cell development in longitude direction; whereas, the blue light strongly inhibited the development (Table 1, Figure 2b). Compared to the control (FL) light, leaf area was dramatically reduced under the red light treatment, whilst the blue light, BRW151, and BRW142 exerted no significant effects. However, only BR was found to strikingly enhance the leaf area (Figure 3). The decreases in leaves enlargement significantly influenced the total biomass accumulation, including fresh weight, dry weight (Figure 5a,b), or dry matter percentages (Table 1). As the above mentions, the obtained results indicated that the material biomass, including the stem cell anatomy of A. roxburghii showed broad variation responses under the different LED light treatments. The relative influence percentage of LED lights on each morphological and development indexes compared to FL were theoretically transformed from the average value when the value of each index of FL was assumed to be 100% (Supplement Data Table S2). Then, the total average of influence of each LED light on A. roxburghii plants was calculated from nine transformed data. Interestingly, we have found that the highest total average influence was in the BR treatment, while a slight inhibition was in the red treatment. All other light treatments showed negligible effects compared with the FL (Table 1; Figure 4).PDF Image | LED Lights Promote Growth and Flavonoid Accumulation of Anoectochilus roxburghii
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