LED Lights Promote Growth and Flavonoid Accumulation of Anoectochilus roxburghii

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LED Lights Promote Growth and Flavonoid Accumulation of Anoectochilus roxburghii ( led-lights-promote-growth-and-flavonoid-accumulation-anoecto )

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Plants 2020, 9, 1344 15 of 15 55. Besseau, S.; Hoffmann, L.; Geoffroy, P.; Lapierre, C.; Pollet, B.; Legrand, M. Flavonoid accumulation in Arabidopsis repressed in lignin synthesis affects auxin transport and plant growth. Plant Cell 2007, 19, 148–162. [CrossRef] 56. Thain, S.C.; Murtas, G.; Lynn, J.R.; McGrath, R.B.; Millar, A.J. The circadian clock that controls gene expression in Arabidopsis is tissue specific. Plant Physiol. 2002, 130, 102–110. [CrossRef] [PubMed] 57. Sun, W.; Shen, H.; Xu, H.; Tang, X.; Tang, M.; Ju, Z.; Yi, Y. Chalcone isomerase a key enzyme for anthocyanin biosynthesis in Ophiorrhiza Japonica. Front. Plant Sci. 2019, 10. [CrossRef] [PubMed] 58. Millar, A. Input signals to the plant circadian clock. J. Exp. Bot. 2003, 55, 277–283. [CrossRef] [PubMed] 59. Nguyen, N.H.; Lee, H. MYB-related transcription factors function as regulators of the circadian clock and anthocyanin biosynthesis in Arabidopsis. Plant Signal. Behav. 2016, 11, e1139278. [CrossRef] [PubMed] 60. Ni, J.; Dong, L.; Jiang, Z.; Yang, X.; Chen, Z.; Wu, Y.; Xu, M. Comprehensive transcriptome analysis and flavonoid profiling of Ginkgo leaves reveals flavonoid content alterations in day–night cycles (Min, X.J., Ed.). PLoS ONE 2018, 13, e0193897. [CrossRef] [PubMed] 61. Furukawa, H. Cultivation technology for vegetable and herb production. In Plant Factory Using Artificial Light Adapting to Environmental Discruption and Clues to Agricultural Innovation; Masakazu, A., Fukuda, H., Wada, T., Eds.; Elsevier Publishing: Cambridge, MA, USA, 2019; pp. 15–23. 62. Easlon, H.M.; Bloom, A.J. Easy leaf area: Automated digital image analysis for rapid and accurate measurement of leaf Area. Appl. Plant Sci. 2014, 2, 1400033. [CrossRef] 63. Marinova, D.; Ribarova, F.; Atanassova, M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J. Uni. Chem. Technol. Metall. 2005, 40, 255–260. 64. Sultana, B.; Anwar, F.; Ashraf, M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 2009, 14, 2167–2180. [CrossRef] 65. Ghasemzadeh, A.; Jaafar, H.; Rahmat, A.; Wahab, P.; Halim, M. Effect of different light intensities on total phenolics and flavonoids synthesis and antioxidant activities in young ginger varieties (Zingiber Officinale Roscoe). Int. J. Mol. Sci. 2010, 11, 3885–3897. [CrossRef] 66. Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25, 402–408. [CrossRef] 67. Yang, L.; Zhang, J.C.; Qu, J.T.; He, G.; Yu, H.Q.; Li, W.C.; Fu, F.L. Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus. Sci. Rep. 2019, 9, 20171. [CrossRef] [PubMed] 68. Zhang, G.; Zhao, M.; Song, C.; Luo, A.; Bai, J.; Guo, S. Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Anoectochilus Roxburghii. Mol. Biol. Rep. 2012, 39, 5905–5912. [CrossRef] [PubMed] 69. Zhang, Y.; Li, Y.; Chen, X.; Meng, Z.; Guo, S. Combined metabolome and transcriptome analyses reveal the effects of Mycorrhizal fungus ceratobasidium Sp. AR2 on the flavonoid accumulation in Anoectochilus roxburghii during different growth stages. Int. J. Mol. Sci. 2020, 21, 564. [CrossRef] [PubMed] 70. Zhang, Y.; Li, Y.; Guo, S. Effects of the Mycorrhizal fungus ceratobasidium Sp. AR2 on growth and flavonoid accumulation in Anoectochilus roxburghii. PeerJ 2020, 8, e8346. [CrossRef] [PubMed] © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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