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60 S.Y. Lee et al. / Journal of Photochemistry and Photobiology B: Biology 88 (2007) 51–67 Fig. 5. In the treatment groups, a significant increase in the amount of collagen was observed by histologic evaluation with standard preparation with H & E (a). This finding was confirmed by Masson-trichrome stain for collagen (b) (x 200 for all). (a) Left: Before treatment, Right: 2 weeks after treatment completion (b) Left: Before treatment, Right: 2 weeks after treatment completion. Fig. 6. A significant increase in the amount of elastic fibers was observed in the treatment groups (Verhoeff-van Gieson stain, x 200). Left: Before treatment, Right: 2 weeks after treatment completion. decreased mildly after 633 nm alone treatment (0.54-fold). After the combination therapy with 830 and 633 nm, how- ever, the levels were 5.39-fold those of the baseline. The pattern of the levels being higher with the use of infrared light was similar to that of TNF-a. The level of ICAM-1 mRNA decreased after sham treatment, 0.4-fold that of the baseline. The changes in the mRNA levels of Cx43 revealed an increase after treatment. They were 1.31, 2.53, and 2.42- fold times those of the baseline after treatment with 830 nm alone, 633 nm alone, and a combination of 830 and 633nm, respectively. Unlike IL-1ß, TNF-a, or ICAM-1, the degree of increase was highest when 633 nm was used alone. The Cx 43 mRNA level was 0.92-fold that of baseline after the sham treatment. 4. Discussion Most nonablative rejuvenation technologies are designed to produce controlled amounts of thermal damage to the dermis, that is, a subclinical wounding, to invoke the wound healing process which leads to biosynthesis of new collagenPDF Image | LED phototherapy for skin rejuvenation
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