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Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts

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Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts ( low-level-laser-therapy-proliferation-and-collagen-synthesis )

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J Wound Manag Res 2018 March;14(1):1-6 https://doi.org/10.22467/jwmr.2018.00283 Journal of Wound Management and Research pISSN 2586-0402 eISSN 2586-0410 Effect of Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts in Vitro Huiling Ma1, Jong-Phil Yang1, Rui Ken Tan1, Hyup-Woo Lee1,2, Seung-Kyu Han1 1Department of Plastic Surgery, Korea University Guro Hospital, Korea University College of Medicine, Seoul; 2Department of Laboratory Medicine, College of Medicine, KangWon National University, Chuncheon, Korea Abstract Background: Low-level laser therapy (LLLT) is a nonthermal technology that can be used to modulate cellular activity through light irradiation at specific pulse sequences. In vitro and in vivo studies have been performed previously to de- termine the effect of LLLT on wound healing. However the results were inconsistent. Final purpose of our project is to determine effect of LLLT on diabetic wound healing and this pilot study was designed to confirm effect of LLLT on activ- ity of healthy human fibroblasts. In particular, we focused on cell proliferation and collagen synthesis, which are main contributing factors in would healing. Methods: Healthy human fibroblasts obtained from cryopreserved cells (n=10) were irradiated at wavelength of 635 nm (RED), 830 nm (IR), and 635 nm+830 nm (FX) with the same fluence of 60 J/cm2, after seeding into 96-well plates. A group in which no laser exposure was applied was assigned as control. Fibroblast proliferation was examined by EZ-Cytox enhanced cell viability assay and immunohistochemistry (IHC). Collagen synthesis was measured by IHC. IHC pictures were analyzed to identify the intensity values of collagen type I as quantity results. Results: Irradiation at FX and IR groups showed a significant increase in fibroblast proliferation and collagen synthesis compared to control and RED groups. There was no significant difference in fibroblast proliferation and collagen syn- thesis between FX group and IR group. Conclusion: Healthy human fibroblasts showed better cell proliferation and collagen synthesis when they were irradi- ated at wavelength of 635 nm+830 nm or 830 nm. Keywords: Fibroblasts, Low-level laser therapy, Wound healing Introduction The prevalence of chronic wounds is increasing dramatically, as the populations of industrialized countries age and become more sedentary. Chronic wounds that re- spond poorly to conventional treatment, making them very difficult to manage [1]. It is well-known that fibroblasts provide desired growth factors and other substances to accelerate wound healing. However, fibroblasts from chronic wounds such as dia- betic ulcers have been commonly demonstrated a lower rate of proliferation when compared with healthy ones [2]. Therefore, it is significant to increase fibroblast ac- tivity for accelerating wound healing. The care of chronic wounds has become its own specialty, with providers often using advanced therapies, including growth fac- tors, extracellular matrices (ECMs), engineered skin, and negative pressure wound therapy (NPWT) [3]. Low-level laser therapy (LLLT) is a nonthermal technology that can be used to modulate cellular activity through light irradiation at specific pulse sequences [4]. In vitro and in vivo studies have been performed previously to determine the effect of LLLT on wound healing. However the results were inconsistent. Using a variety of red, blue, yellow, and infrared light wavelength, it has been reported that LLLT can Original Article Received: January 19, 2018 Revised: February 27, 2018 Accepted: February 27 2018 Corresponding author: Seung-Kyu Han, M.D., Ph.D. Department of Plastic Surgery, Korea University Guro Hospital, Korea University College of Medicine, 148 Gurodong-ro, Guro-gu, Seoul 08308, Korea Tel: +82-2-2626-3333 Fax: +82-2-868-6698 E-mall: pshan@kumc.or.kr This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2018 Korean Wound Management Society www.jwmr.org 1

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