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Int. J. Mol. Sci. 2020, 21, 2370 9 of 20 Figure 5. NIRL and RL regulated gene expression analysis of isolated photoreceptors. (A–E) Late event analysis after 9 h of cultivation. Eyes were treated for 6 h with BL, following 10 min NIRL or RL and post-cultivated for another 3 h. (A) Schematic set up of microdissected photoreceptors via Laser Capture Microdissection (LCM) for gene expression analysis. (B) Venn diagram illustrating overlap of significant differentially expressed genes (DEGs) from C_BL, BL_BL+NIRL 810 nm and BL_BL+RL 670 nm with a cutoff of log2 FC > 0.5 and an adjusted p-value < 0.1 (C) Heatmap of overlapping up- and downregulated genes significant for C_BL and BL_BL+NIRL 810 nm or BL_BL+RL 670 nm ranked by fold change. N = 2 experiments. #—not significant. (D) Representative images for situ hybridization from cryaa and cryab mRNA in retinal sections. N = 3 experiments E: Representative images of CryαA and CryαB immunohistochemical staining in retinal sections. D, E: Retinal layers: outer segments [OS], inner segments [IS], outer nuclear layer [ONL]. N = 3 experiments.PDF Image | Photobiomodulation Mediates Neuroprotection against Retinal
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