PDF Publication Title:
Text from PDF Page: 003
Abstract ACCEPTED MANUSCRIPT Müller cells, the supporting cells of the retina, play a key role in responding to retinal stress by releasing chemokines, including CCL2, to recruit microglia and macrophages (MG/MΦ) into the damaged retina. Photobiomodulation (PBM) with 670nm light has been shown to reduce inflammation in models of retinal degeneration. In this study, we aimed to investigate whether 670nm light had an effect on Müller cell-initiated inflammation under retinal photo-oxidative damage (PD) in vivo and in vitro. Sprague-Dawley rats were pre-treated with 670nm light 2 (9J/cm ) once daily over 5 days prior to PD. The expression of inflammatory genes including CCL2 and IL-1β was analysed in retinas. In vitro, primary Müller cells dissociated from neonatal rat retinas were co-cultured with 661W photoreceptor cells. Co-cultures were exposed to PD, followed by 670nm light treatment to the Müller cells only, and Müller cell stress and inflammation were assessed. Primary MG/MΦ were incubated with supernatant from the co- cultures, and collected for analysis of inflammatory activation. To further understand the mechanism of 670nm light, the expression of COX5a and mitochondrial membrane potential (∆Ψm) were measured in Müller cells. Following PD, 670nm light-treated Müller cells had a reduced inflammatory activation, with lower levels of CCL2, IL-1β and IL-6. Supernatant from 670nm light-treated co-cultures reduced activation of primary MG/MΦ, and lowered the expression of pro-inflammatory cytokines, compared to untreated PD controls. Additionally, 670nm light-treated Müller cells had an increased expression of COX5a and an elevated ∆Ψm following PD, suggesting that retrograde singaling plays a role in the effects of 670 nm light on Müller cell gene expression. Our data indicates that 670nm light reduces Müller cell-mediated retinal inflammation, and offers a potential cellular mechanism for 670 nm light therapy in regulating inflammation associated with retinal degenerations. 3 ACCEPTED MANUSCRIPTPDF Image | Photobiomodulation with 670 nm light ameliorates retinal
PDF Search Title:
Photobiomodulation with 670 nm light ameliorates retinalOriginal File Name Searched:
retinal-degeneration-vs-photobiomodulation-670-nm.pdfDIY PDF Search: Google It | Yahoo | Bing
Cruise Ship Reviews | Luxury Resort | Jet | Yacht | and Travel Tech More Info
Cruising Review Topics and Articles More Info
Software based on Filemaker for the travel industry More Info
The Burgenstock Resort: Reviews on CruisingReview website... More Info
Resort Reviews: World Class resorts... More Info
The Riffelalp Resort: Reviews on CruisingReview website... More Info
| CONTACT TEL: 608-238-6001 Email: greg@cruisingreview.com | RSS | AMP |