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ACCEPTED MANUSCRIPT Figure 1. In vivo expression of cytokines was mitigated by 670nm light treatment following photo-oxidative damage (PD) in rat retinas. A-B: PD significantly increased levels of Il-1β (A) and Ccl2 (B) in retinas compared to dim-reared controls (P<0.05). However, Il-1β and Ccl2 were significantly reduced after 670nm light treatment compared to PD retinas (P<0.05). C-F: Ccl2 in situ hybridization revealed that numerous Ccl2-positive cells (arrows) were present in the INL of PD retinas (E) compared to dim-reared controls (C). After 670nm light treatment, a lower number of Ccl2-positive cells were detected in PD retinas (F). G: 670nm light significantly reduced the number of Ccl2-positive Müller cells in the INL following PD (P<0.05). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. N=5 was used for all experimental comparisons, except for (B) (N=10), and was performed in biological duplicate. The data is presented as the mean ± SEM. * denotes a significant change (P<0.05). Colour should be used 27 ACCEPTED MANUSCRIPTPDF Image | Photobiomodulation with 670 nm light ameliorates retinal
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