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ACCEPTED MANUSCRIPT Figure 2. 670nm light treatment reduced primary Müller cell stress when co-cultured with 661W cells exposed to photo-oxidative damage (PD). A: Timeline of experimental paradigm. B-D: Neither PD nor 670nm light influenced ATP (B), MTT (C) and cell death (D) in Müller cells. E: 670nm light reduced Gfap expression in Müller cells following PD (P<0.05). F: Expression of Rlbp1 was elevated in 670nm-treated Müller cells compared to untreated Müller cells (P<0.05). G-I: Following PD, co-culturing Müller cells (M) with 661W cells increased ATP (G) and MTT (H) and diminished cell death (I) in 661W cells compared to 661W cells cultured alone (P<0.05). ATP, MTT and cell death were not affected by 670nm light treatment. DIV; days in vitro. N=6 was used for all experimental comparisons and was performed in biological triplicate. The data is presented as the mean ± SEM. * denotes a significant change (P<0.05). Colour should be used 29 ACCEPTED MANUSCRIPTPDF Image | Photobiomodulation with 670 nm light ameliorates retinal
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