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ACCEPTED MANUSCRIPT Figure 6. 670nm light ameliorated Müller cell-mediated activation of primary microglia/macrophages (MG/MΦ) following photo-oxidative damage (PD). A: Timeline of experimental paradigm. B-E: After incubation with supernatant (SN) of the PD group (D), MG/MΦ cells displayed an amoeboid morphology. However, a ramified shape was observed in MG/MΦ incubated with SN of the PD+670nm group (E). F-I: A higher mean of fluorescence intensity (MFI) of CD11b (F, G) and CD86 (H, I) was found in MG/MΦ incubated with SN of the PD+670 group compared to SN of the control group (P<0.05). However, MG/MΦ incubated with SN of the PD+670 group had a lower MFI of CD11b and CD86 compared to the PD group (P<0.05). J-O: Reduced gene expression of Ccl2 (J), Il-1β (K), Tnfα (L), Il-6 (M), Il-10 (N), and Sod2 (O) was found in MG/MΦ incubated with SN of the PD+670 group compared to the PD group (P<0.05). DIV; days in vitro. N=6 was used for all experimental comparisons and was performed in biological triplicate. The data is presented as the mean ± SEM. * denotes a significant change (P<0.05). Colour should be used 35 ACCEPTED MANUSCRIPTPDF Image | Photobiomodulation with 670 nm light ameliorates retinal
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