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Int. J. Mol. Sci. 2018, 19, 1107 6 of 17 and with the maximal laser exposure time. Taking the cell viability results together: since MB alone has an intensive, up to 75% toxicity without light exposure, the MB treatment time was considered to be kept as short as possible by high MB concentrations, and the maximal light-dependent cell toxicity was chosen, which was by 8 min laser exposure. The final preferred conditions: 4 min MB exposure with the concentration of 160 μM and 8 min laser exposure, allowed up to 95% reduction of the cell viability in comparison to the control, and 41% of this cell loss was due to light specific effects and not to MB only (Figure 3). Table 2. Phototoxic effect on top of MB-toxicity 660 nm laser exposure after MB pretreatment at different concentrations and times on Detroit 562 cells. MB alone achieved up to 75% cell loss (written in blue). The treatment aim was to decrease the cell viability up to 95% cell loss, which was only achieved in combination of MB and 660 nm laser exposure. The combinations achieved this aim are written in green. The lower part of the table shows the differential effects of 660 nm laser exposure on top of the MB effect. The highest laser-specific effect, which was accompanied with at least 95% cell loss, is written in red. 660 nm Laser MB-Exposure Exposure (min) (min) MB Concentration, μM 40 80 120 160 % of viable cells 0 288696550 0 473566046 0 657413425 0 855444134 2 266544131 2 451393429 2 640141022 2 828231812 4 252393127 4 464443524 4 6301473 4 8211488 6 249383030 6 469553325 6 631684 6 8191476 8 246383627 8 439885 8 626322 8 8191153 2 222162419 2 422162617 2 61727243 2 828202322 4 236303423 4 49122522 4 627272722 4 835293226 6 239313520 6 4502721 6 626352621 6 836303428 8 242313024 8 434475241 8 631383223 8 836323630 Viablility % difference to t = 0 min Laser exposurePDF Image | Photodynamic Low Level Laser Squamous Cell Carcinoma
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