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Cancers 2018, 10, 144 6 of 15 CPE in both HCC1937 and MDA-MB-231 cell lines (23% and 42% cell viability) as compared with either OAdmCherry alone (80% and 78% cell viability) or TMZ alone (75% and 87% cell viability) (FigureCa4nBce)r.s2A01d8,G10F,xPFOdRidPEnEoRtREinVIdEuWcecytotoxicityaloneorincombinationwithTMZ(Figu6roef154A,B). TMZ increased OAdmCherry virus production approximately 10-fold in both cell lines compared to OAdmCherry-infected cells treated with vehicle control DMSO (Figure 4C). Overall, this suggests OAdmCherry-infected cells treated with vehicle control DMSO (Figure 4C). Overall, this suggests that that TMZ increases OAdmCherry-mediated CPE in human TNBC cells via productive virus TMZ increases OAdmCherry-mediated CPE in human TNBC cells via productive virus replication. replication. Figure 4. Temozolomide (TMZ) enhances oncolytic adenovirus (OAd)-mediated cytopathic effect Figure 4. Temozolomide (TMZ) enhances oncolytic adenovirus (OAd)-mediated cytopathic effect (CPE) (CPE) through increased viral replication: (A) Human triple-negative breast cancer (TNBC) cells were through increased viral replication: (A) Human triple-negative breast cancer (TNBC) cells were infected infected with OAdmCherry or adenovirus expressing green fluorescent protein (AdGFP) at a with OAdmCherry or adenovirus expressing green fluorescent protein (AdGFP) at a multiplicity of multiplicity of infection concentration of 2.5 alone or in combination with either dimethyl sulfoxide infectio(nDMcoSnOc)enotraTtMioZn.oAft2.752alhonpeosotriinfecotimonb,incraytsiotanlwviiotlheteisthaienrindgimweatshyulsesudlftoxeidvaelu(DatMeSCOPE).oArTMZ. At72hrepporsetseintfaetcivtieonst,acinryinsgtailsvsihoolewtnstoafintihnrgeeweaxpseurismedentosepvearflouramtedC.P(BE).AOArdep-mresdeiantteadtiCvePEstawiansingis calculated by measuring the absorbance of solubilized dye at 590 nm. Results represent the mean of shown of three experiments performed. (B) OAd-mediated CPE was calculated by measuring the three repeated measurements ± standard deviation (SD; error bars) (* p < 0.05). (C) Supernatants were absorbance of solubilized dye at 590 nm. Results represent the mean of three repeated measurements ± collected and used to determine adenovirus yield from each cell line. Results represent the mean of standard deviation (SD; error bars) (* p < 0.05). (C) Supernatants were collected and used to determine three repeated measurements ± standard deviation (SD; error bars) (* p < 0.05). adenovirus yield from each cell line. Results represent the mean of three repeated measurements ± standard deviation (SD; error bars) (* p < 0.05).PDF Image | Temozolomide Enhances Triple-Negative Breast Cancer Virotherapy
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