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Pharmaceuticals 2020, 13, 137 3 of 9 2. Results Pharmaceuticals 2020, 13, x 3 of 9 2.1. In Vitro PDT with Surface Illumination TLTDL1D413433-3m-medeidaitaetdedPDPDT Twwasaseveavlaulautaetdedwwithithhuhmumananlulnugngadaedneoncoacracricnionmoma a(A(A54594)9)ceclelsll.s. TLTDL1D413433w3wasansonnotnotxoixcictotwowaradrdAA545949cecelllslsoovveerrththeecconccenttrrattiionrangeinvestigatedintheabssencceeof ofaalilgighhttrtereaatmtmeennt.tT.hTehefefeffcetcivtievceocnocnecnetnratrtiaotniorneqreuqirueidretdotroedreudceucelclevlilavbialibtyilibty5b0y%50(E%C(5E0)Cwa)swmausch 50 mgurcehagterreathtearnththaenhthigehheisgthceosntceontcreantitornattioesntetedst(e6d0 μ(6M0 )μ(MFi)g(uFrigeu1r)e. U1)p.oUnpiorrnaidriratdioiantiwonithw5it3h25n3m2 nlimght 2222 lig(h2t8(m28Wm/cWm/c,m20,J2/c0mJ/c)m, th)e, tEhCe5E0 dCropdpreodpptoed0.t0o909.±0909.0±010.μ0M01 yμiMeldyiinegldainpghaotpohthoetorathperuatpiceuintidceixnd(reaxtio (raotfiodaorfkdtaorkligtohtliEgCht50EvCaluevsa)loufes>)>o60f0>.>T6h0e0p. hTohteocpyhtotoxciyctiotytowxiacsityattwenasuatetednbuyatmedorbeythmaonre20t0h-afnold 50 2222 20w0-iftohld63w0i-tnhm63li0g-hntm(6l5igmhtW(6/5cmW,2/3c0mJ/,c2m30),J/wcmhic)h,wrehsiuclhterdesiunltaendEinC5a0nvEaClu50evoafl2u0e.9of±230.5.9μ±M3.(5FμigMure 50 22 (Fi1g)u.rTeh1e).ETCh5e0EdCosedporsoedpurcotdsuocbtstaoibnteadinweditwhigthregerneeannadndrerdedlilgighhttweerre1.98μμM·(·(JJ//ccm))aanndd48408707μMμM 50 (J/(cJm/cm),)r,ersepsepceticvtievlye,lyre,sruesltuinltginignian2a420400-f0o-lfdoldidffiefrfenrecnecbeebtweteweenetnhtehtewtowloiglhigthctocnodnidtiiotniosndsedsepsipteitoenolnyly 22 a 4a04-f0o-fldoldidffiefrfenrecnecienitnhtehneunmumbebreorfopfhpohtotnosnasbasbosrobrebdedbybyTLTDLD1413433atathtehetwtwoodidffieffrernetnwt wavaevlelnegntghtsh.s. Figure 1. Relative cell viability of human lung adenocarcinoma (A549) cells treated with Figure 1. Relative cell viability of human lung adenocarcinoma (A549) cells treated with ruthenium- ruthenium-based photosensitizer (TLD1433). Cell viability was determined by resazurin assay. based photosensitizer (TLD1433). Cell viability was determined by resazurin assay. No exposure to Noexposuretolight(blackboldcircles),irradiationat532-nmlight(greendownwardstrian2gle) light(blackboldcircles),irradiationat532-nmlight(greendownwardstriangle)at28mW/cm for20 at28m2W/cm2for20J/cm2,and630-nmlight(redupwardstrian2gles)at65mW2 /cm2for230J/cm2. J/cm , and 630-nm light (red upwards triangles) at 65 mW/cm for 230 J/cm . Error bars represent Error bars represent standard error of the mean. Cells were treated with the following concentrations of standard error of the mean. Cells were treated with the following concentrations of TLD1433 before TLD1433 before exposure to 532 nm light of 0.01, 0.05, 0.1, 0.3 and 0.5 μM. Similarly, cells were treated exposure to 532 nm light of 0.01 μM, 0.05 μM, 0.1 μM, 0.3 μM and 0.5 μM. Similarly, cells were treated with TLD1433 concentrations of 2, 10, 20, and 40 μM before 630 nm light illumination. TLD1433 only with TLD1433 concentrations of 2 μM, 10 μM, 20 μM, and 40 μM before 630 nm light illumination. (no exposure to light) was assessed at higher concentrations of TLD1433: 0.1, 0.3, 0.5, 1, 5, 10, 30, TLD1433 only (no exposure to light) was assessed at higher concentrations of TLD1433: 0.1 μM, 0.3 and 60 μM. In vitro experiments were completed in triplicate. μM, 0.5 μM, 1 μM, 5 μM, 10 μM, 30 μM, and 60 μM. In vitro experiments were completed in triplicate. The two light conditions exhibited a different irradiance dependence (Figure 2), and similar photocytotoxicity could be obtained between the wavelengths by changing the concentration of TLD1433, the fluence, and the irradiance. Increasing the irradiance to 150 mW/cm2 at 630 nm with 4500 μM (J/cm2) induced a similar photocytotoxicity as 532 nm using an irradiance of 28 mW/cm2 with 14 μM (J/cm2), with no significant toxicity observed when cells were treated with light alone (Figure 2).PDF Image | TLD1433-Mediated Photodynamic Therapy Lung Cancer Cells
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