PDF Publication Title:
Text from PDF Page: 004
Molecules 2011, 16 1392 Figure 2. UV-vis absorption spectra of TαPcZn in DMSO/water (1:3, v/v) mixtures. 2.2. Inhibitory effect of TαPcZn-PDT on the proliferation of Bel-7402 cells and human dermal fibroblasts From the biochemical aspect, an ideal photosensitizer should be non-toxic in the dark but after being exposed to light exhibit potent cytotoxic activity. Although a number of evidences have demonstrated that ideal phthalocyanine photosensitizers-mediated PDT can apparently inhibit proliferation in a variety of cancer cells [7-10,20-24], we firstly report the effect of hydrophilic/lipophilic TαPcZn-PDT on proliferation of Bel-7402 cells by the 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. As shown in Figure 3, the red light (600-700 nm) without TαPcZn did not exhibit any toxicity against Bel-7402 cells, and TαPcZn alone had little effect on the proliferation of Bel-7402 cells. Figure 3. Effect of TαPcZn-PDT on the proliferation of Bel-7402 cells and HDFs. Proliferation of Bel-7402 cells and HDFs analyzed by MTT assay. Bel-7402 cells and HDFs were treated with the different concentrations of TαPcZn in the presence of red-light irradiation (53.7 J/cm2). Cell viability was detected by MTT assay. P < 0.01 versus the control value (cells treated with 0.1% DMSO). 120 100 80 60 40 20 0 Bel-7402 cells treated with 0.1% DMSO/TαPcZn Bel-7402 cells treated with 0.1% DMSO/TαPcZn in the presence of red-light irradiation (53.7J/cm2) HDFs treated with 0.1% DMSO/TαPcZn HDFs treated with 0.1% DMSO/TαPcZn in the presence of red light irradiation(53.7J/cm2) 0μM 18μM 36μM 54μM TαPcZn content (μM) However, TαPcZn-PDT remarkably suppressed the proliferation of Bel-7402 cells in a dose- dependent pattern, indicating that the combination of TαPcZn and red light irradiation can apparently inhibit the proliferation of Bel-7402 cells. The inhibition rate in Bel-7402 cells after TαPcZn-PDT treatment was significantly difference at P < 0.01 compared with the control cells. Furthermore, Figure Cell viability (%)PDF Image | Zinc-Mediated Photodynamic Therapy Inhibits the Proliferation
PDF Search Title:
Zinc-Mediated Photodynamic Therapy Inhibits the ProliferationOriginal File Name Searched:
molecules-16-01389.pdfDIY PDF Search: Google It | Yahoo | Bing
Cruise Ship Reviews | Luxury Resort | Jet | Yacht | and Travel Tech More Info
Cruising Review Topics and Articles More Info
Software based on Filemaker for the travel industry More Info
The Burgenstock Resort: Reviews on CruisingReview website... More Info
Resort Reviews: World Class resorts... More Info
The Riffelalp Resort: Reviews on CruisingReview website... More Info
CONTACT TEL: 608-238-6001 Email: greg@cruisingreview.com (Standard Web Page)