PDF Publication Title:
Text from PDF Page: 007
NASA LED Irradiation and Wound Healing 311 FIG. 11. Cumulative results of data from 11 patients (SEALS) showing improvement in range of motion, pain, and girth reported as percentage change from chronic, unimproving injured baseline after LED treatment at 4 J/cm2, 10 mW/cm2. determined using ELISA (enzyme linked immunosorbent as- say). The changes in the VEGF concentration throughout the 14-day experiment can be seen in Figure 8. The LED (880 nm) group experiences a VEGF peak at day 4 much like the control group. In contrast, the hyperoxic effect of the HBO suppresses the day 4 peak, and instead, the HBO groups peak at day 7. The synergistic effect of the HBO and LED (880 nm) can be seen at day 4. The VEGF level for the group receiving both treatments is markedly higher at day 4 than the HBO only group. The HBO and LED (880 nm) treated group also experiences the day 7 peak characterized by the HBO treatment. Hence, there is a more uniform rise and fall to the VEGF level in the combined treatment group as opposed to the sudden increases seen in the control, LED only, and HBO only groups. By day 14, the HBO- treated groups have dropped closer to the normal level than the LED (880 nm) only or control groups. The synergistic effects of HBO and LED (880 nm) can also be seen easily in Figure 9. The pattern of the changes in basic fibroblast growth factor (FGF-2) concentration is similar to that of the VEGF data. It is clear that the LED (880 nm) day 4 peak is higher than the day 4 peak of the control group. These peaks can be attributed to the hypoxic effect of the tissue ischemia created in the surgery. The hyperoxia of the HBO therapy has a greater effect on suppressing the FGF-2 concentration at day 4 than the VEGF concentration at the same time point. The syn- ergy of the two treatments is evident when looking at the HBO and LED (880 nm) treated group. The concentration of FGF-2 at day 4 is significantly enhanced by the LED (880 nm) treat- ment. Whereas the level would normally drop off by day 7 for a LED-only treated wound, the HBO effect seizes control, caus- ing the concentration of FGF-2 to plateau. Hence, an elevated FGF-2 concentration is achieved throughout the greater part of the 14-day treatment with both HBO and LED (880 nm) thera- pies. Further analysis of the excised wounds will include matrix metalloproteinase2 and 9 (MMP-2 and MMP-9) determination by ELISA, histological examination, and RNA extraction. A wound healing impaired type 2 diabetic mouse model has also been studied. As previously reported, genetically diabetic mice treated with low level laser irradiation demonstrated sig- nificantly enhanced wound closure grossly, and improved wound epithelialization,cellular content, granulation tissue for- mation, collagen deposition, and extensive neovascularization on histological evaluation.21 In our study, type 2 diabetic mice with excisional skin wounds were treated with LEDs at individ- ual wavelengths of 680, 730, and 880 nm at 4 J/cm2 and 50 mW/cm2. LED treatment produced increased healing rates, compared to surgical controls, as seen in Figure 10. A repeated measures analysis was conducted using a general linear model with SqrtArea as the dependent variable and Treat as the independent variable. The interaction effect Day*Treat is significant (p value 5 0.0095), indicating that there is a signifi- cant difference between treatments on some days. This test is of primary interest in this situation, because it shows that the treat- ments are effective for some part of the treatment period (Fig. 10). This analysis was carried out using the SAS statistical soft- ware package, published by The SAS Institute, Inc. TABLE 1. PAIN INTENSITY IN MUCOSITIS PATIENTS IS REDUCED BY LED THERAPY IN CHEEKS COMPARED TO THROAT (CONTROL = 100%) Day 1 Day 2 Day 3 100 100 100 98.56 67.81 84.78 104.1 71.21 81.48 33.2 22.9 20.7 34.2 25.2 20.4 33.8 24 20.6 Day 4 100 79.78 82.32 18.1 23.5 24.1 Day 5 100 86.16 86.82 17.8 24.5 24.3 Day 6 100 63.79 60.93 18.9 24.1 25.9 Day 7 100 52.73 53.27 21.7 22.6 17.9 11 17.7 11.1 Day 8 Day 9 100 61.48 58.65 22.8 18 15.8 Control (throat) Right cheek Left cheek Control SEM Right SEM Left SEM 100 58.65 57.48PDF Image | Effect of NASA Light-Emitting Diode Irradiation on Wound Healing
PDF Search Title:
Effect of NASA Light-Emitting Diode Irradiation on Wound HealingOriginal File Name Searched:
Effect-of-NASA-light-emitting-diode-irradiation-on-wound-healing.pdfDIY PDF Search: Google It | Yahoo | Bing
Cruise Ship Reviews | Luxury Resort | Jet | Yacht | and Travel Tech More Info
Cruising Review Topics and Articles More Info
Software based on Filemaker for the travel industry More Info
The Burgenstock Resort: Reviews on CruisingReview website... More Info
Resort Reviews: World Class resorts... More Info
The Riffelalp Resort: Reviews on CruisingReview website... More Info
| CONTACT TEL: 608-238-6001 Email: greg@cruisingreview.com | RSS | AMP |