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Antibiotics 2020, 9, 98 4 of 12 Antibiotics 2019, 8, x 1 of 12 2.2. In Vitro Photodynamic Inactivation of E. coli Growing in Planktonic State 2.2. In Vitro Photodynamic Inactivation of E. coli Growing in Planktonic State 2 Using 31 μM of MB and 18 J/cm2 of red light, at least a 3 log CFU/mL (99.9%) reduction was Using 31 μM of MB and 18 J/cm of red light, at least a 3 log10 CFU/mL (99.9%) reduction was achieved for all strains and more than 6 log for half of them. Data are shown in Figure 1. Control achieved for all strains and more than 6 log10 for half of them. Data are shown in Figure 1. Control experiments showed that the effect of light alone is negligible for all strains (p > 0.9) while MB alone experiments showed that the effect of light alone is negligible for all strains (p > 0.9) while MB alone exerts some toxicity (<1 log ; p < 0.0001). On the other hand, there were significant differences among exertssometoxicity(<1log100;p<0.0001).Ontheotherhand,thereweresignificantdifferencesamong the control and the PDT-treated groups (p < 0.0001) and there were also significant differences in PDT the control and the PDT-treated groups (p < 0.0001) and there were also significant differences in PDT susceptibility among the strains (p < 0.0001). susceptibility among the strains (p < 0.0001). Figure 1. In vitro photodynamic inactivation of E. coli growing in planktonic state (18 J/cm2 at 625 ± 25 nm, 31 μM MB concentration). Survival fractions are presented as mean + standard deviation Figure 1. In vitro photodynamic inactivation of E. coli growing in planktonic state (18 J/cm2 at 625 ± (SD). Circles: cell control (BS1 -L -MB); triangles: light control (BS2 +L -MB); squares: MB control 25 nm, 31 μ M MB concentration). Survival fractions are presented as mean + standard deviation (SD). (BS3 -L +MB); bars: test (BS4 +L +MB). Circles: cell control (BS1 -L -MB); triangles: light control (BS2 +L -MB); squares: MB control (BS3 -L +MB); bars: test (BS4 +L +MB) In vitro Photodynamic Inactivation of E. coli Growing in Biofilm All the strains studied in this work grew in biofilm. The photodynamic inactivation treatments 2.2.1. In vitro Photodynamic Inactivation of E. coli Growing in Biofilm. using the same conditions employed for the cells in planktonic state were ineffective, therefore the MB All the strains studied in this work grew in biofilm. The photodynamic inactivation treatments concentration was increased. An antimicrobial effect (99.9% or 3 log10 CFU/mL reduction) could be using the same conditions employed for the cells in planktonic state were ineffective, therefore the achieved for all the strains at a concentration of 78.2 μM. Data are shown in Figure 2. MB concentration was increased. An antimicrobial effect (99.9% or 3 log10 CFU/mL reduction) could Control experiments showed that the effect of light or MB alone is negligible for all strains be achieved for all the strains at a concentration of 78.2 μ M. Data are shown in Figure 2. (p > 0.9). On the other hand, there were significant differences among the control and the PDT-treated groups (p < 0.0001) and there were also significant differences in PDT susceptibility among the strains (p = 0.0007).PDF Image | Effective Photodynamic Inactivation of 26 E coli Strains
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