Photobiomodulation Mediates Neuroprotection against Retinal

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Photobiomodulation Mediates Neuroprotection against Retinal ( photobiomodulation-mediates-neuroprotection-against-retinal )

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Int. J. Mol. Sci. 2020, 21, 2370 5 of 20 Figure 2. NIRL and RL inhibit mitochondria induced apoptosis pathway through Bcl-2, Bax and Caspase-9 regulation. (A,B) Late event analysis after 9 h of cultivation. Eyes were treated for 6 h with BL, following 10 min NIRL or RL and post-cultivated for another 3 h. (A) Representative images of Bcl-2, Bax and Caspase-9 (Casp9) immunohistochemical staining in retinal sections. Retinal layers: outer segments [OS], inner segments [IS], outer nuclear layer [ONL]. N = 3 experiments. (B) Western blot analysis of retinal lysates using antibodies against Bcl-2, Bax and Caspase-9 and β-Actin as loading control. Protein level was quantified using ImageJ. N = 6 experiments ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01 compared with blue light.

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