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Int. J. Mol. Sci. 2020, 21, 2370 6 of 20 Figure 3. NIRL and RL increase oxidative phosphorylation in BL irradiated cells. (A–D) Late event analysis after 9 h of cultivation. Eyes were treated for 6 h with BL, following 10 min NIRL or RL and post-cultivated for another 3 h. (A) Representative images of NADH (Complex I) and SDH (Complex II) enzyme activity, labeled on unfixed retinal cryo-sections. Staining represents reduction of NBT to NBT-formazan catalyzed by oxidation of NADH or SDH. Retinal layers: outer segments [OS], inner segments [IS], outer nuclear layer [ONL]. The graph displays quantitative analysis of NADH and SDH relative enzyme activity: By measuring grey values of IS and OS the relative enzyme activity was calculated and normalized to Control (C). N = 5 experiments ± SEM. (B) Table shows oxygen consumption rates in purified OS (0.04 mg protein) for each of the two pathways: OXPHOS I+III+IV and OXPHOS II+III+IV. To stimulate the first pathway 20 mM fumarate was employed and 20 mMPDF Image | Photobiomodulation Mediates Neuroprotection against Retinal
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