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ACCEPTED MANUSCRIPT Figure 4. The effects of 670nm light on activation of the NLRP3 inflammasome in Müller cells following photo-oxidative damage (PD). A: Timeline of experimental paradigm. B-F: Expression of inflammasome genes including Casp8 (B), Nlrp3 (C), Pycard (D), Casp1 (E), and Il-18 (F) were comparable between 670nm-treated and untreated Müller cells following PD (P>0.05). G: Il-1β expression was significantly lowered in 670nm-treated Müller cells compared to untreated Müller cells (P<0.05). DIV; days in vitro. N=6 was used for all experimental comparisons and was performed in biological triplicate. The data is presented as the mean ± SEM. * denotes a significant change (P<0.05). Colour should be used 32 ACCEPTED MANUSCRIPTPDF Image | Photobiomodulation with 670 nm light ameliorates retinal
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