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Appl. Sci. 2020, 10, 2531 15 of 18 References 1. Martino, A.D.; Meichenin, A.; Shi, J.; Pan, K.; Bowler, C. Genetic and phenotypic characterization of Phaeodactylum tricornutum (Bacillariophyceae) accessions1. J. Phycol. 2007, 43, 992–1009. [CrossRef] 2. Depauw, F.A.; Rogato, A.; Ribera d’Alcalá, M.; Falciatore, A. Exploring the molecular basis of responses to light in marine diatoms. J. Exp. Bot. 2012, 63, 1575–1591. [CrossRef] 3. Vejrazka, C.; Janssen, M.; Benvenuti, G.; Streefland, M.; Wijffels, R.H. Photosynthetic efficiency and oxygen evolution of Chlamydomonas reinhardtii under continuous and flashing light. Appl. Microbiol. Biotechnol. 2013, 97, 1523–1532. [CrossRef] 4. Dring, M.J. Photocontrol of Development in Algae. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1988, 39, 157–174. [CrossRef] 5. Furukawa, T.; Watanabe., M.; Shihira-Ishikawa., I. Green- and blue-light-mediated chloroplast migration in the centric diatom Pleurosira laevis. Protoplasm 1998, 203, 214–220. [CrossRef] 6. Holdsworth, E.S. Effect of growth factors and light quality on the growth, pigmentation and photosynthesis of two diatoms, Thalassiosira gravida and Phaeodactylum tricornutum. Mar. Biol. 1985, 86, 253–262. [CrossRef] 7. Acuapan-Hern, J.; Canizares-Villanueva, R.O.; Cristani-Urbina, E. Red Light and Nitrogen Depletion Stimulate the Synthesis of Lipids and N-Alkadienes Susceptible to be Used as Biofuels in Botryococcus braunii UTEX 2441 (Race A). Biotechnol. Indian J. 2017, 13, 155. 8. Kreslavsky, V.D.; Kobzar, E.F.; Muzafarov, E.N. Effect of red radiation, kinetin and linuron on growth and ethylene production in Chlorella. Biol. Plant. 1997, 39, 427–430. [CrossRef] 9. López-Figueroa, F.; Rüdiger, W. Stimulation of Nitrate Net Uptake and Reduction by Red and Blue Light and Reversion by Far-Red Light in the Green Alga Ulva Rigidal. J. Phycol. 1991, 27, 389–394. [CrossRef] 10. Armbrust, E.V.; Berges, J.A.; Bowler, C.; Green, B.R.; Martinez, D.; Putnam, N.H.; Zhou, S.; Allen, A.E.; Apt, K.E.; Bechner, M.; et al. The Genome of the Diatom Thalassiosira Pseudonana: Ecology, Evolution, and Metabolism. Science 2004, 306, 79–86. [CrossRef] 11. Jungandreas, A.; Costa, B.S.; Jakob, T.; von Bergen, M.; Baumann, S.; Wilhelm, C. The Acclimation of Phaeodactylum tricornutum to Blue and Red Light Does Not Influence the Photosynthetic Light Reaction but Strongly Disturbs the Carbon Allocation Pattern. PLoS ONE 2014, 9, e99727. [CrossRef] [PubMed] 12. Wang, H.; Wang, H. Phytochrome Signaling: Time to Tighten up the Loose Ends. Mol. Plant 2015, 8, 540–551. [CrossRef] [PubMed] 13. de Mooij, T.; de Vries, G.; Latsos, C.; Wijffels, R.H.; Janssen, M. Impact of light color on photobioreactor productivity. Algal Res. 2016, 15, 32–42. [CrossRef] 14. Bonente, G.; Formighieri, C.; Mantelli, M.; Catalanotti, C.; Giuliano, G.; Morosinotto, T.; Bassi, R. Mutagenesis and phenotypic selection as a strategy toward domestication of Chlamydomonas reinhardtii strains for improved performance in photobioreactors. Photosynth. Res. 2011, 108, 107. [CrossRef] 15. Kirst, H.; García-Cerdán, J.G.; Zurbriggen, A.; Melis, A. Assembly of the Light-Harvesting Chlorophyll Antenna in the Green Alga Chlamydomonas reinhardtii Requires Expression of the TLA2-CpFTSY Gene. Plant Physiol. 2012, 158, 930–945. [CrossRef] [PubMed] 16. Slattery, S.S.; Diamond, A.; Wang, H.; Therrien, J.A.; Lant, J.T.; Jazey, T.; Lee, K.; Klassen, Z.; Desgagné-Penix, I.; Karas, B.J.; et al. An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum. ACS Synth. Biol. 2018, 7, 328–338. [CrossRef] [PubMed] 17. Chen, W.; Zhang, C.; Song, L.; Sommerfeld, M.; Hu, Q. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J. Microbiol. Methods 2009, 77, 41–47. [CrossRef] [PubMed] 18. Kimura, K.; Yamaoka, M.; Kamisaka, Y. Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence. J. Microbiol. Methods 2004, 56, 331–338. [CrossRef] 19. Bligh, E.G.; Dyer, W.J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 1959, 37, 911–917. [CrossRef] 20. Yu, E.T.; Zendejas, F.J.; Lane, P.D.; Gaucher, S.; Simmons, B.A.; Lane, T.W. Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation. J. Appl. Phycol. 2009, 21, 669. [CrossRef]PDF Image | Red Light Variation Lipid Profiles in Phaeodactylum tricornutum
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