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Molecules 2011, 16 1395 2.5. Effect of TαPcZn-PDT on the cycle of Bel-7402 cells Accumulated results have demonstrated that phthalocyanines-mediated PDT can lead to cancer cell growth inhibition and/or apoptosis through G0/G1 or G2/M cell cycle arrest [22-24]. However, it is unclear whether TαPcZn-PDT can result in growth inhibition and/or apoptosis of Bel-7402 cell through arresting cell cycle. Therefore, in the present study, effect of TαPcZn-PDT on Bel-7402 cell cycle is investigated by DNA flow cytometry analysis. Compared with the control treatment, light treatment alone and TαPcZn treatment alone, TαPcZn-PDT apparently resulted in a decrease in the percentage of cells in G0/G1 and G2/M phase, and an increase in the percentage of cells in S phase (Figure 6), suggesting that TαPcZn-PDT arrested Bel-7402 cells at S phase and subsequently led to a decline in the percentage of cells in G2/M phase. Therefore, we conclude that TαPcZn-PDT inhibits the proliferation of Bel-7402 cell through S cell cycle arrest. Figure 6. Effect of TαPcZn-PDT on the cycle and apoptosis of Bel-7402 cells assayed by flow cytometry analysis of DNA content. Assay of cycle and apoptosis in: panel A, control Bel-7402 cells treated with 0.1% DMSO; panel B, Bel-7402 cells treated with red-light irradiation (53.7 J/cm2); panel C, Bel-7402 cells treated with 54 μM TαPcZn; and panel D, Bel-7402 cells treated with 54 μM TαPcZn in the presence of red-light irradiation (53.7 J/cm2). 2.6. Effect of TαPcZn-PDT on Bcl-2 and Fas in TαPcZn-PDT-induced apoptosis of Bel-7402 cells Bcl-2 is an anti-apoptotic protein. Fas is a 45-kD type II transmembrane receptor protein belonging to the tumor necrosis factor receptor family. Although Bcl-2 and Fas have been documented to play a crucial regulatory role in phthalocyanines-PDT-induced apoptosis [30-32], it is not clear whether Bcl-2 and Fas are involved in TαPcZn-PDT-induced apoptosis of Bel-7402 cells. Therefore, in the present study, effect of TαPcZn-PDT on Bcl-2 and Fas in TαPcZn-PDT-induced apoptosis of Bel-7402 cells is firstly investigated by Immunoblot assay. Compared with the control treatment, light treatment alone and TαPcZn treatment alone, TαPcZn-PDT dose-dependently led to down-regulation of Bcl-2 and Fas in TαPcZn-PDT-induced apoptosis of Bel-7402 cells (Figure 7), suggesting that Bcl-2 and Fas might modulate TαPcZn-PDT-induced apoptosis of Bel-7402 cells.PDF Image | Zinc-Mediated Photodynamic Therapy Inhibits the Proliferation
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