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Data collection and analysis At all sites, date of first open flower was recorded for 12 plants in each treatment that were randomly selected at the beginning of the trial. Additional data were recorded at MSU, such as date of first visible flower bud or inflorescence (VB), number of VB, and main stem length at flowering. For ‘EWBS’ and ‘WPC’ petunia, the stem with the first open flower was measured for stem length. All plants were checked every 1 to 2 d for first flowering except at Kube Pak, where flowering was checked once or twice a week. Days to flower from the start of lighting treatments (and at Kube Pak from when plants were transplanted) and flowering percentage were subsequently calculated for each cultivar in each treatment. The trials ended on 10 Apr. 2013, 4 Apr. 2013, 8 Apr. 2013, 20 Apr. 2013, 4 Apr. 2013, and 24 June 2013, at C. Raker & Sons, CfAHR, Henry Mast Greenhouse, Krueger-Maddux Greenhouses, Kube Pak, and MSU, respectively. The photoperiod from sunrise to sunset, actual average daily temperature (ADT), and photosynthetic daily light integral (DLI) for each site are provided in Table II-1. At C. Raker & Sons, CfAHR, and Henry Mast Greenhouse, a weather station (WatchDog 2400; Spectrum Technologies, Aurora, IL) measured temperature and photosynthetically active radiation. At Krueger-Maddux Greenhouses, a light meter (Lightscout DLI 100 Light Meter; Spectrum Technologies) estimated the DLI, and the grower read instantaneous temperatures with a thermostat and thermometers but did not record actual data. At Kube Pak, a weather tracker (WatchDog 305; Spectrum Technologies) measured temperature and photosynthetically active radiation. At MSU, line quantum sensors (Apogee Instruments, Inc., Logan, UT) positioned horizontally at plant height measured photosynthetically active radiation every 10 s, and a data logger (CR10; Campbell Scientific, Logan, UT) recorded hourly averages. An aspirated thermocouple [36-gauge (0.127-mm diameter) type E] on each bench measured air temperature 37PDF Image | LED REGULATE FLOWERING OF PHOTOPERIODIC ORNAMENTAL CROPS
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